Rapid alkalinization in alfalfa root hairs in response to rhizobial lipochitooligosaccharide signals

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Rhizobium nod factor perception and signalling.

Book. (Madison, Wisconsin: www.medicago.org). Etzler, M.E., Kalsi, G., Ewing, N.N., Roberts, N.J., Day, R.B., and Murphy, J.B. (1999). A Nod factor binding lectin with apyrase activity from legume roots. Proc. Natl. Acad. Sci. USA 96, 5856– 5861. Felle, H.H., Kondorosi, E., Kondorosi, A., and Schultze, M. (1995). Nod signal-induced plasma membrane potential changes in alfalfa root hairs are dif...

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A nuclear-targeted cameleon demonstrates intranuclear Ca2+ spiking in Medicago truncatula root hairs in response to rhizobial nodulation factors.

Lipochitooligosaccharide nodulation factors (NFs) secreted by endosymbiotic nitrogen-fixing rhizobia trigger Ca(2+) spiking in the cytoplasmic perinuclear region of host legume root hairs. To determine whether NFs also elicit Ca(2+) responses within the plant cell nucleus we have made use of a nucleoplasmin-tagged cameleon (NupYC2.1). Confocal microscopy using this nuclear-specific calcium repo...

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A Nuclear-Targeted Cameleon Demonstrates Intranuclear Ca Spiking in Medicago truncatula Root Hairs in Response to Rhizobial Nodulation Factors

Lipochitooligosaccharide nodulation factors (NFs) secreted by endosymbiotic nitrogen-fixing rhizobia trigger Ca spiking in the cytoplasmic perinuclear region of host legume root hairs. To determine whether NFs also elicit Ca responses within the plant cell nucleus we have made use of a nucleoplasmin-tagged cameleon (NupYC2.1). Confocal microscopy using this nuclear-specific calcium reporter has...

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Root hairs.

Roots hairs are cylindrical extensions of root epidermal cells that are important for acquisition of nutrients, microbe interactions, and plant anchorage. The molecular mechanisms involved in the specification, differentiation, and physiology of root hairs in Arabidopsis are reviewed here. Root hair specification in Arabidopsis is determined by position-dependent signaling and molecular feedbac...

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Calcium Spiking in Plant Root Hairs Responding to Rhizobium Nodulation Signals

SUMMARY Rhizobium lipochitooligosaccharide signal molecules stimulate multiple responses in legume host plants, including changes in host gene expression, cell growth, and mitoses leading to root nodule development. The basis for signal transduction in the plant is not known. We examined cytoplasmic free calcium in host root hairs using calcium-sensitive reporter dyes. Image analysis of injecte...

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ژورنال

عنوان ژورنال: The Plant Journal

سال: 1996

ISSN: 0960-7412,1365-313X

DOI: 10.1046/j.1365-313x.1996.10020295.x